Project: PRJNA1209362
This study is a comparative analysis to examine whether there are significant gene expression changes after 6 hours of treatment with three diverse microtubule destabilizers: combretastation A4 (CA4), vinblastine (VB), and plinabulin (PL). Other comparators include baseline control DMSO, microtubule destabilizer docetaxel (DTXL), and inflammatory cytokine TNF-α. We use primary human pulmonary microvascular endothelial cells as a model for a cell sensitive to microtubule perturbations. Overall design: RNA-seq profiling of primary human pulmonary microvascular endothelial cells (HPMECs) treated for 6 hours with either 0.1% DMSO, 1 ng/ml TNF-α, 100 nM combretastatin A4, 100 nM vinblastine, 100 nM plinabulin, or 100 nM docetaxel. Cells from three donors were purchased from PromoCell. Donor identification between the metadata and data file names corresponds as follows: donor 1 = donor 1, donor 2 = donor 4, donor 3 = donor 6.
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