We used GeoMx DSP technology to profile the gene expression information of intraepithelial neutrophils (IENs) and lamina propria neutrophils (LPNs) from moderate-severe UC patients's colon. Overall design: FFPE blockes were obtained from moderate-severe UC patients colon. Slides were processed for GeoMx processing under the manufactor instruction; panCK and Elastase were used to mark the area of interest.