The U. S. Department of Agriculture (USDA)/Agricultural Research Service (ARS) and The Institute for Genomic Research (TIGR) sequenced 26,110 ESTs derived from Aspergillus flavus wildtype strain NRRL3357. They identified 7,218 unique gene sequences, 3,749 of which were assembled from overlapping ESTs and 3,469 of which were singleton ESTs. Based on sequence similarity to known proteins, they assigned putative functions to about 66% of these genes.