Project: PRJNA176416
Transcriptional profiling of the Donna II mixed community containing Dehalococcoides mccartyi strain 195 comparing a batch starved control to the mixed community being fed 1,2,3,4-tetrachlorobenzene as an electron acceptor. The goal was to determine which transcripts are regulated in response to a shift in a different electron acceptor rather than the consistent tetrachloroethene (PCE) that the parent reactor was maintained on. Overall design: Three-condition experiment, triplicate Donna II batch fed 1,2,3,4-tetrachlorobenzene sampled at 2 days vs. pooled Donna II batch fed PCE sampled at 3 days; triplicate Donna II batch fed 1,2,3,4-tetrachlorobenzene sampled at 3 days vs. pooled Donna II batch fed PCE sampled at 3 days . The additional two microarrays are the technical dye-swap of the 3 day starved control.