Wild type or Pax6 fx/fx; Le-Cre-positive (Pax6-/-) surface ectoderm from E9.5 mouse embryos was laser micodissected from three embryos of each genotype. Total RNA was purified and reverse transcribed and amplified using a NuGEN kit. cDNA was biotinylated and hybridized to Illumina Mouse6 bead arrays. Overall design: Three wild type and three knockout embryos were used. Each array used the cDNA obtained from the two prospective lens tissues (left and right eyes) from each embryo.