In our previous work, we had found that Saccharomyces cerevisiae needs of the Hog1 and Slt2 proteins to growth in a low pH environment caused by sulfuric acid, one of the stress factors during the process of ethanol production. Then was performed the gene-wide expression analysis in the hog1∆ and slt2∆ mutants in order to reveal the function of the Hog1p and Slt2p MAP Kinases in the regulation of S. cerevisiae global gene expression upon stress by sulfuric acid. Overall design: BY4741 strain (Reference) and their derivate mutants hog1∆ and slt2∆ were grown for 1 h in YPD medium pH 2.5 adjusted with concentrated sulfuric acid