We screened unialgal isolates obtained from hypersaline ecosystems in the southwest United States and identified this strain of Tetraselmis as the most halotolerant phototroph characterized to date that is capable of both robust fermentative and photo H2 production. This halophilic species of Tetraselmis was isolated from the Great Salt Lake in Utah, and appears to be particularly promising for biotechnological applications in saline systems. Additionally, this strain was included in the Moore Foundation’s marine eukaryotic algal transcriptome project and is among the first species of Tetraselmis with a publicly available transcriptome. We would like to submit our data to make it more widely available to the research community for further investigation.