Monterey Bay is a long-term ecological research site, with the efforts of several major research institutions focused on understanding the Bay's oceanography. Our lab has generated large-insert genomic libraries from genomic fragments of Monterey Bay microbes. These libraries have been characterized extensively, both phylogenetically through surveys of rDNA genes and functionally through surveys of genes of particular interest (e.g. proteorhodopsin, bacteriochlorophyll, rubisco). Many clones have been fully-sequenced, and each library has been end-sequenced. This project illuminates the community diversity, structure, and ecosystem functional potential of a coastal marine microbial community. In addition, monthly cruises since 1998 have sampled and archived microbial community DNA from Monterey Bay, and the genomic libraries provide vital context for interpreting the longer-term seasonal and annual changes in these communities.

Concentrated picoplankton cells from Monterey Bay, California, USA.

Four environmental genomic libraries were made from four depths: EB000: 3m depth, Station M2 (36.7°N, 122.4°W), collected 3/17/99.

BAC vector used, 8352 total clones, 80kb average

The following BAC submissions are associated with this project: AE008919-AE008921, AF268611, AF279106, AY372454, AY458629-AY458650, EF089397-EF089400, EF106972, EF107099-EF107100, and EF107104-EF107105