The SPRUCE experimental site, or S1 bog (8.1 ha), is located in northern Minnesota, USA within Marcell Experimental Forest (MEF: N 47°30.476´; W 93°27.162´). This predominantly raid-fed, ombrotrophic bog has an average pH of 4.1 at the surface that increases with depth to an average value of 5.1 at 2 m. The SPRUCE project involves an ecosystem-scale climate manipulation in S1 Bog. The experimental design includes ten 12 m diameter chambers that are warmed to 5 temperatures (+0, +2.25, + 4, +6.75, and +9 °C), with duplicate plots subjected to ambient and ~ + 850 ppmv CO2. In the most novel aspect of this experiment, the peat is warmed throughout the peat column to depths of 2 - 3 m. Intact soil core samples were collected from 11 depth intervals (0-10, 10-20, 20-30, 30-40, 40-50, 50-75, 75-100, 100-125, 125-150, 150-175 and 175-200 cm) at each of the 10 SPRUCE experimental plots in June 2014 and June 2015. The diversity and composition of prokaryotic communities was determined by applying a high-throughput sequencing-based protocol that targets PCR-generated amplicons from V4 variable regions of the 16S rRNA gene using the bacterial primer set 515F (5′-GTGCCAGCMGCCGCGGTAA-3′) and 806R (5′-GGACTACHVGGGTWTCTAAT-3′)