In this study, two diploids, T. dactyloides var. meridionale (2n = 36, MR) and T. dactyloides (2n = 36, DD), and one tetraploid, T. dactyloides (2n = 72, DL) were sequenced by low-coverage genome sequencing followed by graph-based cluster analysis. The results showed that 63.23%, 59.20%, and 61.57% of the respective genome of MR, DD, and DL were repetitive DNA sequence. The proportions of different repetitive sequences varied greatly among the three species