IMR90 ER:RAS cells were transfected with scramble siRNA or 2 deconvoluted siRNAs targeting each of 38 candidate genes or positive control siRNAs targeting the senescence regulators p16 and p53 and the SASP regulators NF-kappaB, p38alpha and CEBPbeta. The next day the cells were treated with 4OHT to induce senescence. 6 days later the cells were collected for total mRNA analysis. Our previous experiments had shown that these 38 genes regulate the secretome of senescent cells without affecting other senescence phenotypes such as the growth arrest. By performing RNA-seq we confirmed that knockdown of these genes affected the expression of multiple pro-inflammatory factors in senescence. More importantly, the secreted factors were differentially regulated across the different knockdown conditions indicating potential for specific manipulation of immune response genes. Overall design: 92 conditions were examined: proliferating cells (Noninduced), senescent cells (Water, Allstars), senescent cells transfected with 2 siRNAs targeting 38 different genes (Hs_GeneID_n), senescent cells transfected with positive control siRNAs (p16, p53, CEBPB, p38, p65) and additional conditions included proliferating and senescent IMR90 ER:RAS cells mock-transfected or transfected with various non-targeting siRNAs (Extras1-8) to monitor changes in gene epression independent of gene knockdown.