Keratoconus (KTCN) is a complex degenerative eye disorder resulting in loss of visual function. It is known, that its development is affected by both genetic and environmental or behavioral components. In order to verify if DNA methylation may also play a role in KTCN development, reduced representation bisulfite sequencing (RRBS) of human corneas obtained from five KTCN and five non–KTCN individuals was performed. Overall design: DNA methylation analysis of 5 KTCN and 5 non-KTCN corneas using RRBS