In order to identify regions of the genome that display quantitative changes in acetyl-CoA, we utilized an established “spike-in” normalization method that allows the quantitative comparison of histone modifications across cell populations using defined quantities of a reference epigenome. We found that some regions of the genome are more sensitive to changes in acetyl-CoA compared to others. Overall design: ChIP-Seq of histone H3K27ac in glioblastoma samples treated with different amounts of glucose or acetate with a drosophila cell line as the reference epigenome spike-in.