Sterol regulatory element-binding protein 2 (SREBP2) is a transcription factor that has been recently discovered to mediate vascular endothelial cell (EC) dysfunction. We therefore investigated the role of SREBP2 in the epigenetic function of EC biology through comparing ATAC-seq of human umbilical vein endothelial cells (HUVECs) that were infected with adenovirus overexpressing SREBP2 (Ad-SREBP2) or with empty vector (Ad-null) control. Gene ontology analysis revealed that SREBP2 not only decondenses chromatin for cholesterol biosynthesis, but also mediates the TGF pathway. Among the responsive promoter, SREBP2 exhibited the induction of genes related to mesenchymal transition. Overall design: ATAC-seq data of HUVECs overexpressing SREBP2 using adenovirus followed by deep sequencing, in biological duplicate, using Illumina HiSeq 2000.