Clostridium butyricum, a Gram-positive, strictly anaerobic spore-forming bacillus, is a common human and animal gut commensal bacterium, also frequently found in the environment. Clostridium Butyricum JKT37 was isolated from palm oil mill effluent (POME), which was collected from Sime Darby East Oil Mill, Selangor, Malaysia. This microorganism can be utilized for biofuel and biochemical productions from various carbon sources.