Increasing evidence suggests the liver as to be an effector against blood-stage malaria. Vaccination induces changes in the liver and survival of otherwise lethal blood-stage malaria of Plasmodium chabaudi which is associated with changes in the liver. Here, the time-course of expression of erythroid genes is investigated during infections with P. chabaudi in the liver of vaccination-protected and unprotected non-vaccinated mice. Overall design: Female Balb/c mice were vaccinated with a non-infectious vaccine at week 3 and week 1 before challenging with 106 P. chabaudi-parasitized erythrocytes. Gene expression microarrays and quantitative real-time PCR were used to compare mRNA expression of erythroid genes in the liver of vaccinated and non-vaccinated mice at different phases of infections on days 0, 1, 4 , 8, 11 p.i.. 30 samples were analyzed: Nd0, Non-vaccinated day 0, 3 replicates Nd1, Non-vaccinated day 1, 3 replicates Nd4, Non-vaccinated day 4, 3 replicates Nd8, Non-vaccinated day 8, 3 replicates Nd11, Non-vaccinated day 11, 3 replicates Vd0, Vaccinated day 0, 3 replicates Vd1, Vaccinated day 1, 3 replicates Vd4, Vaccinated day 4, 3 replicates Vd8, Vaccinated day 8, 3 replicates Vd11, Vaccinated day 11, 3 replicates