Next generation sequencing (NGS) results demonstrate the modulative capacity of LCPUFAs on dysregulated miRNA expression in asthma. Methods: Sequencing of miRNA was performed by NGS from lung tissue of asthmatic and control mice with normal diet, as well as of LCPUFA supplemented asthmatic mice using Illumina miSeq. Conclusion: Our results demonstrate the modulative capacity of LCPUFAs on dysregulated miRNA expression in asthma. Overall design: Lungs of LCPUFA-treated asthmatic C57BL/6 mice and respective controls were analyzed with n=4-5 via NGS