Amino acids license Treg cell function by priming and sustaining TCR induced mTORC1 activity and Rag and Rheb GTPases as central regulators of mTORC1 activation in effector Treg (eTreg) cells RNA was purified from CD25+ Treg cells isolated from Cd4CreRragafl/flRragabfl/fl or Cd4CreRhebfl/flRhebl1–/– mice or their littermate controls. Overall design: In total 20 samples were analyzed: WT (3 Unsimulated and 3 Stimulated); Rheb1/2 KO (3 Unsimulated and 3 Stimulated) and additional 8 samples of Tregs (2 WT and 2 RagAB KO unstimulated) and (2 WT and 2 RagAB KO Tregs stimulated for 8 hours).