To profile nucleosome free regions of moust palatal epithelium at E14.5, we performed ATAC-seq for isolated palatal epithelium and mesenchyme cells. Overall design: We digested the mouse embryonic palate at E14.5 with 0.25% trypsin-EDTA (Life Technologies) at 4°C for 10 minutes, after which the reaction was stopped using trypsin Inhibitor (Life Technologies). Under a dissecting microscope, the epithelium was isolated for ATAC-seq by gently peeling using microforceps. The remaining tissue, comprised largely of mesenchymal cells, was also collected for control samples. For each biological replicates, 20000 cells from 3 different mouse embryos were used. Three biological replicates were sequenced using HiSeq X Ten.