Transcriptome analysis of total RNA samples from HEK293-PIGS-KO and HEK293-PIGS-UBE2J1-DKO cells. To check whether KO of UBE2J1 upregulates genes of GPI biosthesis pathway, we used microarrays to analyze gene expression change by KO of UBE2J1 and comfirmed that known GPI pathway genes are not changed by ERAD-deficiency. Overall design: HEK293-PIGS-KO and HEK293-PIGS-UBE2J1-DKO cells were collected for total RNA extraction by RNeasy Mini Kit, and microarray analysis on the Clariom S Array, Human.