To reveal the transcriptomic changes during the sex differetiation in larval zebrafish, we treated the lavarl fish with aromatase inhibitors (EM, CAS: 107868-30-4, 100 μg/L), as well as solvent as a control, and sampled them after the exposure. Overall design: PolyA+ RNA-seq were performed on 6 samples, each of which was a mixture of 25 juvenile zebrafish. There are twor groups of larval zebrafish were exposed for two exposure treatments. Each group contained 3 replicates, with 25 fish per replicate. The exposure treatments included (1) DMSO (10 μL/L), (2) EM + DMSO. The exposure periods were 32 days for zebrafish.