An immortalized normal epithelial cell line of common bile duct origin (HBDEC2 cells) was established. An ELF3 gene knockdown clone was obtained using microRNA-155-based conditional RNA interference targeting the human ELF3 gene (5′-AGGTTCTGCTGGATCAGCT-3′) and EGFPmiR, targeting EGFP as a control (EGFP-miR; 5′-ACAAGCTGGTACAACTACA-3′). Overall design: We performed a genome-wide expression profiling analysis of ELF3 KD cells and control cells. Three independent samples were performed.