Single cell RNA-Seq was performed on ID1 ID3 null endothelial cells to determine the effects of ID gene loss on endothelial cell maintainence and growth. Overall design: 20,000 SECs and type H vessels from CDH5-CreER ID1+/+ ID3+/- or CDH5-CreER ID1-/- ID3-/- mice were sorted using FACs and subjected to single cell RNA-Seq.