Improved sequencing and transcriptome analysis are new technologies provided a means to monitor genome-wide changes in transcriptomes to study expression patterns under salinity. IRNA-seq, as an important strategy in the investigation of the expression of a large number of genes in a given tissue at a given time point, has been widely used to research the responses of plants to abiotic stress, particularly salt stress.

This technology can reveal candidate genes and key pathways involved in salt tolerance by analyzing differentially expressed genes (DEGs) and RNA-seq functional annotation. RNA- seq technology assists researchers to establish a better understanding of molecular networks in plants in response to salt stress.