Examples: histone, BN000065

Project: PRJNA658693

We isolated a Nannochloropsis oceanica LHCR mutant, named hlr1, which exhibits a greater tolerance to high light (HL) stress compared to the wild type.Global gene expression of WT and the hlr1 mutant, as a function of time in dark incubation (DK) and under high irradiance was measured by mRNA-Seq Overall design: Total RNA of the algal cells was prepared using an RNA miniprep kit (CWBIO) and the concentrations were measured using the NanoDrop 2000 spectrophotometer (Thermo). Libraries were constructed and then sequenced using the HiSeq 2500 (Illumina), which generated about 18 million read pairs per sample (ANNOROAD). Raw reads containing adapter, poly-N, and low-quality reads were filtered and the effective data were mapped with the Nannochloropsis reference genome using TopHat (version 2.0.12). After excluding the ribosomal RNA or transfer RNA, we estimated the abundance of the transcripts using RPKM (Reads per Kilo bases per Million reads) 62. The p values were adjusted using the Benjamini and Hochberg method 63. Corrected p value﹤0.05 and fold change more than two were set as the threshold for significant difference in expression. GO annotations of the data provided by our RNA-Seq analysis were performed using PANTHER (www.pantherdb.org/pathway/) 64. Three biologically independent sets of samples were prepared at each time point.

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