To determine the molecular signaling pathways responsible for USP16 regulation of cell growth, RNA sequencing was conducted on NCI-H23 cells with or without USP16 knockdown Overall design: Human lung tumor cells NCI-H23 were transfected with control siRNA or USP16 siRNA. The gene expression profiling in NCI-H23 cells with or without USP16 knockdown was analyzed by RNA-seq.