Maternal methionine supply during late pregnancy can affect offspring growth and development via nutritional programming. In the present study, we investigated if increasing the supply of methionine during late-pregnancy in Holstein cows affects transcriptome profiles in the offspring. Overall design: Liver biopsies were harvested at 4 days of age from 6 calves per treatment born to cows fed a control diet or the control plus ethyl-cellulose rumen-protected methionine for the last 28 days prepartum, and were fed and managed similarly after birth