Tumor derived vesicles (TEVs) promote Angpt2 expression in Ch25h-/- ECs, while Reserpine reversed TEV induced Angpt2 expression in ECs. Overall design: Ch25h null ECs isolated from Ch25h k.o mice and pre-treated with Reserpine(10μM for 8hr) followed by PBS or MC38 derived TEVs treatment (20ug/ml) for 12 hr in vitro.