Purpose: Characterize the gene expression profile of knocked-down ZEB1 and ZEB2 H9 cells during myogenic differentiation through RNA sequencing Methods: RNA sequencing of RNA from cell cultures treated for 2 days with CHIR from the three genotypes used in this study: H9shCTL, H9shZEB1 and H9shZEB2 Results: Knock-down of ZEB1 and ZEB2 in H9 cells results in differential gene expression upon differentiation. Conclusions: Compairson of knocked-down ZEB1 and ZEB2 H9 cells display differences in gene expression at day 2 of the differentiation protocol. Overall design: RNA sequencing of total RNA from H9 shCTL, H9 shZEB1 and H9 shZEB2 cells at day 2 of the differentiation protocol. One sample of each genotype from one independent differentiation experiment (3 experiments).