Examples: histone, BN000065

Project: PRJNA719377

In an attempt to understand the mode of antiviral action of lactoferrin on SARS-CoV-2 infection, we focused on the effect of lactoferrin on uninfected cells. We decided to use a physiologically relevant cell model of iPSC derived- alveolar epithelial cells (the iAEC2). We specifically focused on antiviral pathway triggered by lactoferrin on treated cells that could explain a cell-mediated effect of lactoferrin on viral replication Overall design: We seeded iAEC2 cells in collagen-treated plate and allowed them to adhere. When the cells reached confluence, they were treated with lactoferrin at a concentration of 6.25uM (500ug/ml) for two days. Cells were then harvested in TriZol, RNA was extracted with the DirectZol kit (Zymogen) according to manufacturers instructions and was sequences with the Illumina NovaSeq 6000 System.

General