To screen the related miRNAs in the occurence and development of sebaceous gland carcinoma(SGC) of the eyelid , we have employed Human Agilient microarray expression profiling as a discovery platform to identify differentially expressed miRNAs for SGC and TP53 mtation related miRNAs. Human formalin-fixed paraffin-embedded (FFPE) SGC tissues and para-carcinoma sebaceous gland(SG) FFPE samples from SGC patients were screened using microarray assay. Human FFPE SGC tissues containing TP53 mutation and human FFPE SGC tissues without TP53 mutation were screened using microarray. The overlap of two sets of microarray differentially upregulated miRNAs were selected as our research object. Overall design: SGC of eyelid tissues with TP53 mutation contained four repeat samples and SGC of eyelid tissues without TP53 mutation also contained four repeat samples for each experiment.Differentially expressed miRNAs between human SGC of eyelid tissus and para-carcinoma tissues were screened ,and eight repeat samples were performed for each experiment.