The Hippo Pathway has been found to play an important role in adrenal gland development and maintenance in mice. In this study, we further explored the role of the Hippo pathway in the adrenal gland using a transgenic mouse model where we knocked out the upstream kinases of the pathway, Mst1 and Mst2. Overall design: Nr5a1-Cre and Mst1(flox/flox);Mst2(flox/flox) were selectively bred over generations to obtain Mst1(flox/flox);Mst2(flox/flox);Nr5a1-Cre mutant mice. Controls are considered the Mst1(flox/flox);Mst2(flox/flox) mice. At 2 months of age, mice were dissected and adrenal glands were isolated and conserved at -80°C.