In order to profile the transcription events that were regulated by RBM4, mRNA-seq was performed on KYSE150_shRBM4 and KYSE150_ctl which based on KYSE150 cells Overall design: This study includes a total of 2 profiling samples,KYSE150_shRBM4 was generated as a RBM4 knocked down esophageal cancer cells . KYSE150_ctl was generated as a control esophageal cancer cells .