SARS-COV-2 caused COVID-19 has caused unprecedented global health crisis. Coronavirus have developed a variety of mechanisms to interfere host translation machinery to facilitate virus protein production. Here, we conducted Ribometh-seq to explore the effects of ectopic expression of SARS-COV-2 N protein on rRNA 2'-O-me modification profiles of host cells. Overall design: Examination of rRNA 2’-O-me modification in HEK293T cells with or without ectopic expression of SARS-COV-2 N protein.