Project: PRJNA833339
Whole genome sequencing (WGS) of MCF-10A cells without or with NFIB overexpression cultured at 30 or 75 days and 5-fluoracil-resistant or oxaliplatin-resistant MCF-10A cells with NFIB overexpression cultured at 75 days to investigate whether the oncogenic potential of NFIB is linked to its function in replication initiation. Overall design: MCF-10A clones stably overexpressing NFIB via lentivirally delivered NFIB expression vector. These clones were further cultured 30 or 75 days and NFIB overexpression MCF-10A cells under the treatment of oxaliplatin or 5-fluoracil at 75 days being subjected to whole genome sequencing.
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