Skin homeostasis is guided by spatiotemporal regulation of gene expression, establishing each stage of keratinocyte differentiation. The current study presents transcriptomic and chromatin profiling for the proliferating (basal) and differentiated (suprabasal) cell populations derived from neonate mice (p0-p2) skin epidermis. This multi-omic approach will enable idenitification of cell specific epidermal cross-talk central to the equlibirum. Overall design: Chromatin profiling: Chromatin accessiility and chromosome conformation profiling in basal and suprabasal fractions of skin epidermis; Histone and transcription factor occupancy profiling for suprabasal cells in mouse neonate skin epidermis