Human engineered CRC organoids were equipped with the intestinal stem cell reporter STAR reflecting transcriptional activity of ASCL2. Bulk RNA-sequencing was performed on STAR+ and STAR- cells after 5 days and after 12 days. Overall design: Organoids were grown from STAR+ cells (APCKO/KO; KRASG12D/-; TP53KO/KO (AKP)). After 5 days and after 12 days, organoids were harvested, dissociated to single cells, and between 10-20 k STAR+ and STAR- cells were collected in technical replicates for bulk RNA-seq.