Bulk RNA-Seq of sorted B cell populations revealed glucocorticoid pathway being coordinated with B-cell development. Bulk RNA-Seq of human BCP-ALL cell lines treated with dexamethasone identified pathways of resistance. Overall design: Comparative gene expression profiling analysis of RNA-seq data for B-cell sorted populations in 3 heathy donors and cell lines (NALM6, REH wt and REH GCR) treated with vehicle or dexamethasone.