To investigate the mechanism of NPR1 regulating vascular function, we established NPR1 knockout heterozygous mice by CRISPR-Cas9. We performed high-throughput RNA sequencing to analyse the transcriptom of the aorta of NPR1 knocout heterogeneous mice. Overall design: RNA-seq performed in mouse whole aorats from wild type and NPR1 knockout heterozygous mice, n=3 per experimental group, using the BGISEQ-500 platform.