Examples: histone, BN000065

Project: PRJNA906980

Current methods for R-loop mapping need to perform DNA:RNA immunoprecipitation for each sample individually, with consequent limitations in throughput. Here, we develop and validate mDRIP-seq, a multi-sample barcoding and pooling method for R-loop mapping. We show mDRIP-seq performs equivalently as conventional methods, but with the merits of high throughput and cost-efficiency. We also show the simplicity of mDRIP-seq for relative and absolute quantitation of genomic R-loop fractions for multiple samples. Together, mDRIP-seq is a high-throughput and cost-efficient method for R-loop mapping and quantitative assessment and can be widely applied to large-scale dynamic profiles of these important structures for diverse organisms. Overall design: mDRIP-seq development, and R-loop mapping in multiple samples

Secondary Study Accession:
SRP410660
Study Title:
mDRIP-seq: a high-throughput method for R-loop mapping and quantitative assessment
Center Name:
Guangdong Laboratory of Lingnan Modern Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences
Study Name:
mDRIP-seq: a high-throughput method for R-loop mapping and quantitative assessment
ENA-REFSEQ:
N
PROJECT-ID:
906980
ENA-FIRST-PUBLIC:
2024-06-17
ENA-LAST-UPDATE:
2025-02-28
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