To examine the microbiota abundance difference, we performed fecal 16s sequencing of wild type, TCRb-/-, TCRb-/- co-housed with WT and TCRb-/- receiving WT T cells. Overall design: Male WT and TCRb-/- mice were either self-maintained or co-housed for 4 weeks. Fecal pellets were collected for 16S sequencing targeting the V3-V4 region.