Next-generation sequencing (NGS) was used to analyze gene expression in H. pylori CrdRS two-component system deletion strains. The goal of this study was to examine the role that the CrdRS two-component system plays in H. pylori gene expression through identification of regulon differences Overall design: Analysis of transcriptional changes in H. pylori strains with CrdRS two-component system deletions compared to a control A total of 6 independent samples were analyzed; 2 replicates of RNA preparations from H. pylori 26695/ΔrdxA for comparison to the 2 replicates of RNA preparations from H. pylori 26695/ΔrdxA/ΔcrdS and 2 replicates of RNA preparations from H. pylori 26695/ΔrdxA/ΔcrdR