To investigate the role of ChREBPα in the development and progression of non-alcholic fatty liver disease, we investigated how Chrebpα deficiency or overexpression affect the development and progression of NAFLD. Overall design: We compared the gene expression profiling of RNA-seq data from the livers of Chrebpα-flox/flox mice injected with AAV-TBG-GFP (Chrebpα-WT) and AAV-TBG-Cre (Chrebpα-LKO) and fed NASH diet (40 kcal% Fat (Either Palm Oil), 20 kcal% Fructose and 2% Cholesterol) for 12 weeks, and compared the gene expression profile of the livers of wildtype mice injected with Adenovirus overexpressing GFP (Ad-GFP) and Chrebpα (Ad-Chrebpα) and fed HFLMCD diet (45 kcal% Fat, Without Choline and With 0.1% Added Methionine).