H2Aub Chip-seq was undertaken in rats with myocardial ischemia injury to derive a list of signaling pathways and genes Overall design: Ischemia was observed when the left anterior descending (LAD) coronary artery was ligated around the PE-10 tube with moving nodule with 6-0 sutures, and occlusion of the left anterior descending coronary artery was confirmed by elevation of the ST segment of the electrocardiogram.