To characterized a new model for lens development, a 3’ end RNA-seq was perform on the cell in 2D culture, on the all organoids (3D) and on the external and internal part of the organoids obtain with laser microdissection follow by RNA-extraction Overall design: Differential Gene Expression analysis of RNA-seq data from cell (21EM15) on 2D culture, on the all organoids (3D) and on the external and internal part of the organoids was performed