Interrogating Staphylococcus aureus transcriptional changes in the presence or absence of glycosaminoglycans, specifically heparin. Overall design: USA400 bacterial suspensions were grown in the presence of unfractionated heparin (2 µg/mL ) or saline control. After 3 or 10 hours of incubation, bacterial RNA was isolated and sent for sequencing (n=3 per treatment group and time point).