RNA sequencing was performed on three biological replicates of DMSO treated CART19 (control) and TP0903 treated CART19 produced from normal donors. Overall design: T cells from normal donors were transduced with a replication-incompetent lentiviral vector expressing a second-generation chimeric antigen receptor (CAR) consisting of an anti-CD19 single chain variable fragment (FMC63) fused to 4-1BB and CD3ζ intracellular domains as previously described. DMSO or TP-0903 treated CART19 were stimulated with CD19+ cell line Jeko-1 for 24 hours and CART19 were isolated with CD20 microbeads. After confirming CART19 isolation was performed successfully with flow cytometry (>99% purity), RNA was isolated from CART19.