To investigate the changes in mRNA stablity upon loss of LARP1, we performed SLAM-seq and half-life measurements on WT and derived LARP1 KO cells. The canonical and 5′TOP mRNA cell lines used for single-molecule imaging were treated as biological replicates for the SLAM-seq analysis. Overall design: WT cells (canonical and 5′TOP mRNA cell lines) and derived LARP1 KO clones were analyzed by RNA-seq, using independent replicates (cells harvested on different days).