In search for biological control agents, forty-four bacterial strains isolated from greenhouse beet roots were screened for antagonistic activity against the plant parasitic root-knot nematode (RKN) Meloidogyne incognita, which causes significant yield losses in a number of important crops worldwide. Through a combination of in vitro and on planta screening assays, Pseudomonas spp. 105 and 108 were identified as the most promising bacterial isolates and were subsequently sequenced and de-novo assembled.