Purpose: RNA-seq analysis of gene expression patterns in Naïve t, Th0, Treg and Th17 cells of wild-type mice. The goals of this study are to compared the gene expression between Naïve T and other types Th cells by RNA-seq analysise. Overall design: Naïve T cells were CD4+ T cells directly selected from the spleen. Th0 cells were stimulated with αCD3/CD28 for three days. Th17 cells were collected at 3 days under Th17 differentiation conditions, Treg cells were collected at 3 days under Treg differentiation conditions,and then subjected to RNA seq.